XFe 96 Extracellular Flux Analyzer - Seahorse
To use the Seahorse machine please contact the platform to arrange the meeting for discussion of the experimental strategy. Certain cell types will requier the initial tests for an assay optimisation (cell type, cell number, concentrations of the inhibitors). Users can be trained to perform measurements in autonomous mode.
Measurements of Mitochondrial respiration (basal, maximal (FCCP treatment) and spare respiratory capacity (Rotenone and Antimycin A treatment), Glycolytic function (glycolysis, glycolytic capacity (Oligomycin treatment), glycolytic reserve (2 Deoxy-D-glucose treatment) or Fatty acid oxidation
Necker site (INEM, Imagine) and any INSERM affiliated lab - 149 Eur per late (consumables and reagents are provided by the platform)
Any Academic lab - 211 Eur per plate (consumables and reagents are provided by the platform)
Metabolite analyses using Q Exactive Plus Orbitrap Mass Spectrometer
Sample preparation, LC/MS, and data analysis methods vary widely depending on metabolite, sample type, and biological question. To initiate the project the user has to contact the platform to arrange the meeting to discuss the strategies of the experimental design and sample preparation.
Targeted analyses (~100 metabolites per run) per sample:
Necker site (INEM, Imagine) - 26 Eur
INSERM - 38 Eur
Any other academic lab - 46 Eur
Untargeted analyses (includes 4 runs per sample (positive and negative mode, pHILIC and C18 columns) per sample:
Necker site (INEM, Imagine) - 71 Eur
INSERM - 135 Eur
Any other academic lab - 162 Eur