Platform for metabolic analyses provides the services for the metabolic measurements in vitro and in vivo. The aim is to integrate metabolic studies in fundamental research projects to assess cellular and organism physiology. Our project stems from the need to share the equipment and expertise of the labs at Paris Descartes University, Imagine and INEM Institutes by creating a common facility and research network. An important goal is to acquire state-of-the-art equipment.

 

 

Metabolic platform can help to assess metabolism on different levels:

 

Contact

To contact the platform please write an email to Ivan Nemazanyy - ivan.nemazanyy@inserm.fr

Ivan NEMAZANYY  01 40 61 54 86 
-  
Mario PENDE  01 40 61 53 43 
Référent scientifique  
 

XFe 96 Extracellular Flux Analyzer can simultaneously interrogate the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a microplate format, in real-time.

 

Q Exactive Plus benchtop Orbitrap mass spectrometer, equipped with an Ion Max source and a HESI II probe (Thermo Scientific). This system is capable of measuring mass-to-charge ratios with less than 5 ppm error and a resolution of up to 140,000 and can also collect MS/MS fragmentation data. Mass spectrometer is coupled to a Dionex UltiMate 3000 ultra-high performance liquid chromatography (UPLC).

 

 

 

Platform for Metabolic Analyses

Pièce 120
Faculté de Médecine Paris Descartes 

156 rue de Vaugirard

75015

 
XFe 96 Extracellular Flux Analyzer - Seahorse
 

To use the Seahorse machine please contact the platform to arrange the meeting for discussion of the experimental strategy. Certain cell types will requier the initial tests for an assay optimisation (cell type, cell number, concentrations of the inhibitors). Users can be trained to perform measurements in autonomous mode.

 

Services:

Measurements of Mitochondrial respiration (basal, maximal (FCCP treatment) and spare respiratory capacity (Rotenone and Antimycin A treatment), Glycolytic function (glycolysis, glycolytic capacity (Oligomycin treatment), glycolytic reserve (2 Deoxy-D-glucose treatment) or Fatty acid oxidation

 

Charges:

Necker site (INEM, Imagine) and any INSERM affiliated lab - 149 Eur per late (consumables and reagents are provided by the platform)

Any Academic lab - 211 Eur per plate (consumables and reagents are provided by the platform)

 
Metabolite analyses using Q Exactive Plus Orbitrap Mass Spectrometer
 

Sample preparation, LC/MS, and data analysis methods vary widely depending on metabolite, sample type, and biological question. To initiate the project the user has to contact the platform to arrange the meeting to discuss the strategies of the experimental design and sample preparation.

 

Charges :

Targeted analyses (~100 metabolites per run) per sample:

Necker site (INEM, Imagine) - 26 Eur

INSERM - 38 Eur

Any other academic lab - 46 Eur

Untargeted analyses (includes 4 runs per sample (positive and negative mode, pHILIC and C18 columns) per sample:

Necker site (INEM, Imagine) - 71 Eur

INSERM - 135 Eur

Any other academic lab - 162 Eur