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2006 Doctoral thesis: Approaches to vaccine target candidates identification against nontypeable Haemophilus influenzae causing otitis media, Boston University School of Medicine, Boston, MA, USA & University Paris XI, France
2003 DEA Structural, Functional and Evolutionary Approach to Genomics, Doctoral school: Genes, Genomes, Cells; University Paris XI, France
2001 Maîtrise (MS equivalence) Cellular Biology and Physiology, Molecular and Cellular Genetics, University of Texas at Austin, TX, USA & University Paris XI, France
2000 Licence (BS equivalence) Cellular Biology and Physiology, University Paris XI, France
1999 DEUG Natural Sciences, University Paris XI, France
• Other diploma:
2003 GRE General Test
1999 TOEFL
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01/2007 - present Post-Doctorale Research - Role of intestinal microbial flora on innate immunity maturation and consequences on pathogen susceptibility and aging in the nematode Caenorhabditis elegans
Laboratory of Evolutive and Medical Molecular Genetics, INSERM U571, Necker School of Medicine, Paris, France; supervisor: Dr. Ivan Matic
09/2003 09/2006 Approaches to vaccine target candidates identification against nontypeable Haemophilus influenzae causing otitis media. Division of Pediatric Infectious Diseases, Section of Molecular Genetics, Boston University School of Medicine, Boston Medical Center, USA; supervisor: Dr. Richard Goldstein
01/2003 - 08/2003 Development of a metagenomics approach to bacteriophage populations study within biofilm of environmental samplesInstitute of Genetics and Microbiology, UMR 8621, Laboratory of Genetics and Biodiversity of Microbial Biofilms, University Paris XI, Orsay, France; supervisor: Dr. Michael DuBow
11/2002 - 12/2002 Expression of human carcinoma-involved genes in Saccharomyces cerevisia Institute of Genetics and Microbiology, UMR 8621, Laboratory of Molecular Genetics of Mitochondrial Biogenesis and Regulatory Networks, University Paris XI, France; supervisor: Dr. Monique Bolotin-Fukuhara
10/2000 - 05/2001 Evolution of fX type bacteriophage to single and multiple inhibitors Institute of Molecular and Cellular Biology, Laboratory of Integrative Biology, University of Texas in Austin, USA; supervisor: Dr. James Bull
06/2000 - 08/2000 Mechanisms et dynamics of genetic diversity in pollen population of Fraxinus excelsior L., Oleaceae Institute of Ecology, Systematics and Evolution, UMR 8079, Laboratory of Biodiversity, Systematics and Evolution, University Paris XI, France; supervisor: Dr. Pierre-Henry Gouyon
05/1999 07/1999 Screening of Agrobacterium tumefaciens-generated mutants of Arabidopsis thaliana for identification of a malate-dihydrogenase NADP dependant (MDH-NADP) impaired clone. Institute of Plant Biotechnology, UMR 8618, University Paris XI, France; supervisor: Dr. Marianna Stein
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08/2008 Cold Spring Harbor Laboratory Course on C. elegans, August 9-24 2008.
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- Goldstein, R., V. Bouchet, M. Leroy, H. Huot, M. Figueira, S. Ram & S. Pelton. Top-down bottom-up phylogenic approach for early stage vaccine development. Accepted for publication in Recent Advances in Otitis Media, October 2008
- Lundström S. L., J. Li, M. Månsson, M. Figueira, M. Leroy, R. Goldstein, D. W. Hood, E. R. Moxon, J. C. Richards & E. K. H. Schweda. Application of CE-ESI-MS and LC-1 ESI-MSn to profile glycoform expression during Haemophilus influenzae pathogenesis in the chinchilla model of experimental otitis media. Accepted for publication Infect. Immun., May 2008. doi:10.1128/IAI.01710-07
- Leroy M., M. Prigent, M. Dutertre, F. Confalonieri & M. DuBow. 2008. An Appraisal of Bacteriophage Morphotype and Genome Diversity of a Seine River Sediment in Paris. Freshw. Biol., 53(6):1176-1185
- Leroy M., H. Cabral, M. Figueira, V. Bouchet, H. Huot, S. Ram, S. Pelton & R. Goldstein. 2007. Multiple consecutive lavage reveals greater burden of disease while providing direct access to nontypeable Haemophilus influenzae biofilm in experimental otitis media. Infect. Immun., 75(8): 4158-4172
- Prigent M.* and Leroy M.*, F. Confalonieri, M. Dutertre & M. DuBow. 2005. A Diversity of Bacteriophage Forms and Genomes can be Isolated from the Surface Sands of the Sahara Desert. Extremophiles, 9(4): 289-296 (* - authors contributed equally to this work)
- Goldstein, R., V. Bouchet, M. Leroy, H. Huot, M. Figueira, S. Ram & S. Pelton. Top-down bottom-up phylogenic approach for early stage vaccine development. Submitted to Recent Advances in Otitis Media, January 2008.
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Goldstein, R., V. Bouchet, M. Leroy, H. Huot, M. Figueira, S. Ram, S. Pelton. Top-down bottom-up phylogenic approach to early stage vaccine development. 9th International Symposium on Recent Advances in Otitis Media, St Pete Beach, FL, USA, June 3-7, 2007.
Huot, H., V. Bouchet, M. Leroy, S. Pelton, K. O’Brien, R. Reid, M. Santosham, K. O’Neill, M. Lipsitch & R. Goldstein. Congruence of pneumococcal population structure resolved by ribotyping and MLST in a defined community. Fifth International Symposium on Pneumococci and Pneumococcal Diseases (ISPPD5), Alice Spring, Australia, April 2-6, 2006.
Leroy, M., V. Bouchet, H. Huot, S. Pelton & R. Goldstein. Population biology-based strategy for identification of conserved antigens. Cold Spring Harbor laboratory meeting on microbial pathogenesis and host response, Cold Spring Harbor, NY, USA, September 14-18, 2005.
Pelton S., M. Figueira, R. Zanjani, V. Bouchet, M. Leroy, R. Goldstein & S. Ram. Role of complement in experimental otitis media in initial and recurrent infection due to Nontypeable Haemophilus influenzae. Otitis media symposium, Amsterdam, Netherlands, April 24, 2005.
Chen J., V. Bouchet, H. Huot, M. Leroy & R. Goldstein. The potential of population genetics: how a Pseudomonas tree can be used to look for vaccine targets and suggest health policy for cystic fibrosis patients. Fourth annual medical student summer research scholars symposium, Boston University School of Medicine, Boston, MA, USA, January 19, 2005.
Leroy, M., H. Huot, V. Bouchet, M. Figueira, D. Hoods, J. Richards, R. Moxon, S. Pelton & R. Goldstein. Microbial population genetics as the missing link to functional genomics-based vaccine development. American Society for Microbiology conference on functional genomics and bioinformatics approaches to infectious disease research, Portland, OR, USA, October 6-9, 2004.
Huot, H., V. Bouchet, M. Leroy, S. Pelton & R. Goldstein. Implications for vaccine development based on pneumococcal population structure. Fourth International Symposium on Pneumococci and Pneumococcal Diseases (ISPPD4), Helsinki, Finland, May 9-13, 2004
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• Languages: French/English, fluent
• Molecular Biology: DNA, RNA and protein isolation, northern-, southern- and western-blots (radioactivity: 32P, chemoluminescence), cloning (electroporation / bacterial cells transformation, ligations), RT-PCR and RT-PCR quantitative (qRT-PCR), transfection and expression systems, microscopy (Nomarski, Phase, Fluorescence)
• Computer and softwares: PC and Mac, UNIX and DOS (basics), Office Suite (Word, Excel, PowerPoint), Adobe Photoshop, Suite DNAStar (MegAlign, SeqEdit, PrimerSelect), Oligos design softwares (Beacon Designer, Primer Designer, PrimerSelect), EndNote, Gene Construction Kit, PrismGraph
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