Claude SAINT-RUF

	Claude SAINT-RUF
		Research Engineer claude.saint-ruf@inserm.fr tel: +33 (0)1 40 61 53 26 - fax: +33 (01 40 61 53 22 Génétique Moléculaire Evolutive et Médicale U1001 INSERM, Faculté Necker-Enfants Malades 156 rue de Vaugirard, 75730 Paris Cedex 15, France

Education

1982: Deug Science de la vie, University of Tours
1983: Bachelor (License) of cellular biology and physiology, University of Paris VI
1984: Master (Maîtrise) of cellular biology and physiology, University of Paris VI1
1985: Graduate studies (DEA) in Molecular biology of development, University of Paris VI.
April 1989: Ph.D (Doctorat) in Molecular biology of development, University of Paris VI, awarded with highest honors.

Scientific positions

1985-1989- Doctoral research fellow in Laboratory of Cellular Genetics; Director: Roland Cassingena- Institut de Recherche sur le Cancer, Villejuif, France. (Fellowship from the French Research Ministry (Allocataire de recherche).
Subject : Analysis of the malignant conversion of the breast epithelial cell line HBL-100.

1989:1992 - Post-doctoral research fellow in Laboratory of Chromosomal Structure and Mutagenesis; Director: Bernard Dutrillaux.- Institut Curie, Paris, France. (Grants from ARC (Association de Recherche contre le Cancer), and from la Fondation pour la Recherche Médicale ; France)
Subject: Molecular approach of chromosomal mutagenesis in human tumors.

1993-2000- Research Engineer (Ingénieur de recherche, IR), Laboratory of Lymphocyte Development, INSERM U373, Director: Harald von Boehmer - Faculté de Médecine Necker, Paris, France.
Subject: Development of T lymphocytes - Characterization of the pre-T cell receptor.

2000-2009: IR1, Laboratory of Medical and Evolutionary Molecular Genetics, INSERM U571, Director: Miroslav Radman - Faculté de Médecine Necker, University René Descartes-Paris V, Paris, France.
Subject : Stress responses and aging in Escherichia coli colonies.

2010- to date : IR Hors Classe, INSERM U1001 (exU571) Director: Ivan Matic
Faculté de Médecine Necker, University René Descartes-Paris V, Paris, France.
Subject: Bacterial adaptive radiation in structured environment.

Training courses and collaborations

1993- Training courses in Harald von-Boehmer’s laboratory - Basel Institute for Immunology, Switzerland. Immunology and flow cytometry.

1997- Two months stay for a collaboration in Hidde Ploegh’s laboratory, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA. Expression in vitro of pre-T cell receptor.

2000- Cours de Microbiologie (Masters 2)- Institut Pasteur – Paris, France.

Award

Saint-Ruf C., Cordier C. Mégret Jerôme, Matic I, Microbial Stress from molecules to systems. Semmering, Austria. 2009, May 7^th to 10^th.
Spatiotemporal pattern of death and stress associated phenotypes inside aging Escherichia coli colonies.
Prize winner for the best poster (First Prize).

Publications

Saint-Ruf C, Cordier C, Mégret J, Matic I. Reliable detection of dead microbial cells using fluorescnet hydrazides
Appl Environ Microbiol. 2010, 76 (5): 1674-1678

Saint-Ruf C, Ungewiss K, Groettrup M, Bruno L, Fehling HJ, von Boehmer H.
Analysis and expression of a cloned pre-T cell receptor gene.(This paper, first published in Science in 1994, was selected to be reprinted as a Pillar of Immunology article by The Journal of Immunology) J. Immunol. 2009, 182(9): 5165-9.

Touchon M, Hoede C, Tenaillon O, Barbe V, Baeriswyl S, Bidet P, Bingen E, Bonacorsi S, Bouchier C, Bouvet O, Calteau A, Chiapello H, Clermont O, Cruveiller S, Danchin A, Diard M, Dossat C, Karoui ME, Frapy E, Garry L, Ghigo JM, Gilles AM, Johnson J, Le Bouguénec C, Lescat M, Mangenot S, Martinez-Jéhanne V, Matic I, Nassif X, Oztas S, Petit MA, Pichon C, Rouy Z, Saint-Ruf C, Schneider D, Tourret J, Vacherie B, Vallenet D, Médigue C, Rocha EP, Denamur E.
PLoS Genet. 2009 Jan;5(1):e1000344. Epub 2009 Jan 23.

Saint-Ruf C, Pesut J, Sopta M, Matic I. Causes and consequences of DNA repair activity modulation during stationary phase in Escherichia coli.
Critical review in biochemistry and molecular Biology, 2007; 42 (4): 259:70.

Saint-Ruf C., Matic I. Environmental tuning of mutation rates.
Environ Microbiol. 2006; 8(2): 193-9.

Saint-Ruf C, Taddei F and Matic I Stress and survival of aging Escherichia coli rpoS colonies.
Genetics 2004;168: 541-546

Saint-Ruf C., Panigada M., Azogui O., Debey P., von Boehmer H., Grassi F. Different initiation of the pre-TCR and gamma delta TCR signaling.
Nature 2000; 406 (6795): 524 - 7

von Boehmer H, Aifantis I, Feinberg J, Lechner O, Saint-Ruf C, Walter U, Buer J, Azogui O. Pleiotropic changes controlled by the pre-T-cell receptor.
Curr Opin Immunol. 1999;11(2):135-42

Saint-Ruf C, Lechner O, Feinberg J, von Boehmer H.
Genomic structure of the human pre-T cell receptor alpha chain and expression of two mRNA isoforms.
Eur J Immunol. 1998;28(11):3824-31.

Von Boehmer H, Aifantis I, Azogui O, Saint-Ruf C, Grassi F.
The impact of pre-T-cell receptor signals on gene expression in developing T cells.
Cold Spring Harb Symp Quant Biol. 1999;64:283-9

Von Boehmer H, Aifantis I, Azogui O, Feinberg J, Saint-Ruf C, Zober C, Garcia C, Buer J.
Crucial function of the pre-T-cell receptor (TCR) in TCR beta selection, TCR beta allelic exclusion and alpha beta versus gamma delta lineage commitment.
Immunol Rev. 1998;165:111-9.

Aifantis I, Azogui O, Feinberg J, Saint-Ruf C, Buer J, von Boehmer H.
On the role of the pre-T cell receptor in alphabeta versus gamma delta T lineage commitment.
Immunity. 1998;9(5):649-55.

Del Porto P, Bruno L, Mattei MG, von Boehmer H, Saint-Ruf C.
Cloning and comparative analysis of the human pre-T-cell receptor alpha-chain gene.
Proc Natl Acad Sci. 1995 ;19;92(26):12105-9.

Fehling HJ, Krotkova A, Saint-Ruf C, von Boehmer H.
Crucial role of the pre-T-cell receptor alpha gene in development of alpha beta but not gamma delta T cells.
Nature. 1995 29;375(6534):795-8.

Fehling HJ, Laplace C, Mattei MG, Saint-Ruf C, von Boehmer H.
Genomic structure and chromosomal location of the mouse pre-T-cell receptor alpha gene.
Immunogenetics. 1995;42(4):275-81.

Saint-Ruf C, Ungewiss K, Groettrup M, Bruno L, Fehling HJ, von Boehmer H.
Analysis and expression of a cloned pre-T cell receptor gene.
Science. 1994 ; 18;266(5188):1208-12.

Saint-Ruf C, Viegas-Pequignot E, Alfani E, Clementi M, Dutrillaux B, Carloni G.
Co-amplification of transcriptionally active epidermal growth factor receptor and ribosomal genes in the human hepatoma cell line Li7A.
Oncogene. 1992;7(8):1557-65.

Saint-Ruf C, Malfoy B, Scholl S, Zafrani B, Dutrillaux B.
GST pi gene is frequently coamplified with INT2 and HSTF1 proto-oncogenes in human breast cancers.
Oncogene. 1991 ;6(3):403-6.

Saint-Ruf C, Gerbault-Seureau M, Viegas-Pequignot E, Zafrani B, Malfoy B, Dutrillaux B.
Recurrent homogeneously staining regions in 8p1 in breast cancer and lack of amplification of POLB, LHRH, and PLAT genes.
Cancer Genet Cytogenet. 1991;52(1):27-35.

Kokalj-Vokac N, Saint-Ruf C, Lefrancois D, Viegas-Pequignot E, Lemieux N, Malfoy B, Dutrillaux B.
A t(X;15)(q23;q25) with Xq reactivation in a lymphoblastoid cell line from Fanconi anemia.
Cytogenet Cell Genet. 1991;57(1):11-5.

Saint-Ruf C, Gerbault-Seureau M, Viegas-Pequignot E, Zafrani B, Cassingena R, Dutrillaux B.
Proto-oncogene amplification and homogeneously staining regions in human breast carcinomas.
Genes Chromosomes Cancer. 1990; 2(1):18-26.

Saint-Ruf C, Nardeux P, Cebrian J, Lacasa M, Lavialle C, Cassingena R.
Molecular cloning and characterization of endogenous SV40 DNA from human HBL-100 cells.
Int J Cancer. 1989 15;44(2):367-72.

Krief P, Saint-Ruf C, Bracke M, Boucheix C, Billard C, Billard M, Cassingena R, Jasmin C, Mareel M, Azzarone B.
Acquisition of tumorigenic potential in the human myoepithelial HBL100 cell line is associated with decreased expression of HLA class I, class II and integrin beta 3 and increased expression of c-myc.
Int J Cancer. 1989 15;43(4):658-64.

Saint-Ruf C, Nardeux P, Estrade S, Brouty-Boye D, Lavialle C, Rhim JS, Cassingena R.
Accelerated malignant conversion of human HBL-100 cells by the v-Ki-ras oncogene.
Exp Cell Res. 1988, 176(1):60-7.

Marlhens F, Saint-Ruf C, Nardeux P, Lavialle C, Brouty-Boye D, Dutrillaux B, Cassingena R.
Karyotype evolution of the human HBL-100 cell line and mapping of the integration site of SV40 DNA.
Ann Genet. 1988;31(2):81-6.

Lacroix JC, Azzouz R, Saint-Ruf C, Dourdon C, Charlemagne J. Sample chromosomes and identification of sexual chromosomes in Urodeles. Archives d’Anatomie Microscopique et de morphologie Experimentale, 1985 ; 74 (4) 272

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