Anamarija Stafa

Post-Doc

tel: +33 (0)1 44 41 25 26 -
anamarija.stafa@inserm.fr

Inserm U1001 / Centre de Recherches Interdisciplinaires Bat. Faculte Cochin, 2eme étage
24, rue du Faubourg Saint Jacques
75014 PARIS, FRANCE

EDUCATION

2009. PhD. in Molecular Genetics, University of Zagreb, PhD. thesis: "Genetic side effects during gene replacement in the yeast Saccharomyces cerevisiae" (Mentor: prof Z. Zgaga, PhD.)
2003. MSc. of Biotechnology, University of Zagreb; MSc. Thesis: "Transformation of the yeast Saccharomyces cerevisiae strain FFmre11 by integrative plasmids with short terminal heterologies" (Mentor: prof Z. Zgaga, PhD.)

Foreign languages: English (good knowledge), German (intermediate knowledge), French (basic knowledge)



PROFESSIONAL EXPERIENCE


02/2010.-present Postdoctoral research -"Interdisciplinary approaches to aging and death cellular lineages" (INSERM U1001, Evolutionary Systems Biology Group, project leader F. Taddei PhD.)
11/2009.–present Postdoctoral research -"Palindromes in the genomes and mechanisms of gene targeting in yeast" (University of Zagreb, project leader prof. Z. Zgaga, PhD.)
01/2007.–11/2009. Junior researcher - "Palindromes in the genomes and mechanisms of gene targeting in yeast" (University of Zagreb, project leader prof. Z. Zgaga, PhD.)
12/2003.–12/2006. Junior researcher - "Recombination mechanisms in the yeast Saccharomyces cerevisiae" (University of Zagreb, project leader prof. Z. Zgaga, PhD.)


PUBLICATIONS

- Lisnic, B., Svetec, I.-K., Stafa, A., Zgaga, Z. (2009) Size-dependent palindrome-induced intrachromosomal recombination in yeast. DNA repair. 8(3):383-389
- Svetec, I.-K., Stafa A., Zgaga Z. (2007). Genetic side effects accompanying gene targeting in yeast: the influence of short heterologous termini. Yeast 24(8):637-652.
- Stafa, A., Svetec I.-K. Zgaga Z. (2005). Inactivation of the SGS1 and EXO1 genes synergistically stimulates plasmid integration in yeast. Food. Technol. Biotechnol. 43: 103-108.



POSTERS AND SHORT TALKS

Stafa, A.; Ni, M.; Xu L.; Chen, Y.; Lindner, A.B.; Taddei, F. Quantifying mortality curves of individual yeast cells during chronological ageing//Molecular Genetics of Aging// Austad, S.; Campisi J.; Sinclair D.(ed.). Cold Spring Harbor, USA, 2010. 137 (poster)
Stafa, A.; Ni, M.; Xu L.; Chen, Y.; Lindner, A.B.; Taddei, F. Mortality curves of individual yeast cells during chronological ageing//Tor, caloric restriction and anabolism in ageing, MedILS, Split, Croatia, 2010. (short talk)
Stafa, A.; Miklenic, M.; Lisnic, B.; Svetec, I.-K.; Zgaga, Z. The efficiency of gene replacement in yeast is allele-specific//EMBO Young Scientist Forum. Zagreb, Croatia 2009.
Lisnic, B.; Svetec, I.-K.; Stafa, A.; Zgaga, Z. Recombinogenic potential of palindromic sequences is determined by their size and position in the yeast genome//50 Years of Molecular Biology in Croatia / Zahradka, K.; Plohl, M.; Ambriovic-Ristov, A. (ed.). Zagreb:Croatia, 2008. 14-14.
Stafa, A.; Miklenic, M.; Lisnic, B.; Svetec, I.-K.; Zgaga, Z. Targeted chromosome duplication as a side-effect of gene targeting in yeast//50 Years of Molecular Biology in Croatia / Zahradka, K.; Plohl, M.; Ambriovic-Ristov, A. (ed.).Zagreb Croatia, 2008. 58-58.
Stafa A., Miklenic M., Svetec I.-K., Lisnic B., Zgaga Z. Genetic duplications as a side-effect of targeted gene replacement in yeast//Book of abstracts of 38th Annual Meeting of European Environmental Mutagen Society / Franekic-Colic J., Garaj-Vrhovac, V. (ed.) Cavtat, Croatia 2008.
Lisnic, B., Svetec., I.-K., Stafa A., Zgaga Z. Critical size for palindrome induced intrachromosomal recombination in yeast//Book of abstracts of 38th Annual Meeting of European Environmental Mutagen Society / Franekic-Colic J., Garaj-Vrhovac, V. (ed.) Cavtat, Croatia 2008.
Svetec I.-K., Stafa A., Zgaga Z. Genetic duplications as a side-effect of gene replacement in yeast//Book of abstracts of ESF-EMBO Symposium on Comparative Genomics of Eukaryotic Microorganisms: Eukaryotic Genome Evolution / Sant Feliu de Guixols, Spain, 2007.
Zgaga Z., Stafa A., Lisnic B., Beneti L., Svetec I.-K. Ends-out recombination as a source of genetic duplications in Yeast//Book of abstracts of Central European Symposium on Industrial Microbiology and Microbial Ecology / Kosalec, I., Pigac J., Vujaklija D.,(ed.). Zagreb Croatia, 2007.
Lisnic, B.; Svetec, I.-K.; Stafa, A.; Zgaga, Z. Palindromes in prokaryotic genomes//Book of abstracts of The Congress of the Croatian Society of Biochemistry and Molecular Biology on the occasion of the 30th Anniversary / Kovarik, Z. (ed.). Zagreb, Croatia 2006.
Stafa, A.; Lisnic, B.; Svetec, I.-K.; Zgaga, Z. Inactivation of the SGS1 and EXO1 genes synergistically stimulates the efficiencies of yeast transformation//Book Of Abstracts Of The Second Congress Of Croatian Geneticists / Franekic Colic, J.; Ugarkovic, D. (ed.). Zagreb, Croatia 2005.
Lisnic, B.; Stafa, A.; Svetec, I.-K.; Zgaga, Z. Palindromic sequences in the Saccharomyces cerevisiae genome//Book Of Abstracts Of The Second Congress Of Croatian Geneticists / Franekic Colic, J.; Ugarkovic, D. (ed.). Zagreb, Croatia, 2005.


AWARDS

2007. "Outstanding poster presentation" award ESF-EMBO Symposium on Comparative Genomics of Eukaryotic Microorganisms: Eukaryotic Genome Evolution, Sant Feliu de Guixols, Spain, 2007.
2003. Rector’s award for the best student research, University of Zagreb, Croatia (mentor prof. Z. Zgaga PhD.)

ATTENDED COURSES AND SUMMER SCHOOLS:

12.-19.08.2007. EURYI@MedILS Symposium, MedILS, Split, Croatia
25.-29.06.2007. Practical Course "Bioinformatics: Computer Methods in Molecular Biology" ICGEB, Trieste, Italy
02.-05.04.2007. Theoretical Course "RNA structure and function", ICGEB, Trieste, Italy
10.-12.10.2006 "1st Young Scientists’ Brainstorming Meeting", MedILS, Split, Croatia
30.07.-11.08.2006. "Creating Interdisciplinary Research projects", MedILS, Split, Croatia



TEACHING ACTIVITIES:

2004.–2010. Teaching assistant; laboratory courses on "Molecular Genetics", "Genetic Engineering"; co-mentoring several bachelor and master thesis, University of Zagreb, Croatia
2010 Co-lecturer on course "Biology refresher", Interdisciplinary Approaches to Life Sciences ('AIV') Master Program, University Paris Descartes, France

OTHER ACTIVITIES:

2010. Co-organiser of the Summer School of Science 2010 (S3++ 2010; 18.07.-29.07.), Visnjan, Croatia (http://s3.sci.hr/ )
2009. Project leader "The Ames test - mutations in reverse gear", Summer School of Science (S3++2009), Visnjan, Croatia
2007. Workshop leader "Yeast Saccharomyces cerevisiae", Summer Science Factory, MedILS, Croatia

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