IFR Necker-Enfants Malades (IRNEM – IFR 94) was replaced with SFR Necker on January 1st, 2014. It is officially recognized by four public institutions, namely AP-HP, CNRS, INSERM and University Paris Descartes. SFR Necker is entrusted with the development and optimization of technological platforms, as well as contributing to scientific activities on Necker/Broussais site.

To date, 11 platforms are operational and some of them are accessible to researchers and partners institutions from Ile de France Region.


SFR Necker future events

  • Executive committee meeting: December 13th at Imagine
  • SFR Necker annual seminar:  November 29th 2018 at Imagine

 


Recent acquisitions

  • Cytometry core facility, site Broussais --> Spectral analyzer SP6800 SONY

The SP6800 is a very intuitive analyzer cytometer, intended for the autonomous users.
The spectral technology optimizes the sensibility and improves the detection of low signals by collecting the photons from 420nm to 800nm. The spectral technology simplifies the design of multi-panels  colors, by eliminating band-pass filters and the conventional matrices of compensation.
The implemented spectral deconvolution makes the simpler and easier analysis by separating the individual spectral imprints of each fluorochrome. We obtain a more complete image of the rare populations. The complexities associated with the work with fluorescent proteins and fluorochromes excited by several lasers are decreased

Localisation : Broussais, LRB119 – contact : corinne.cordier@inserm.fr

 

  • Cytometry core facility, site Broussais --> Upgrade of the cell sorter in version BD FACS Aria IIIu

This new version improves the systems fluidic, computing and especially optics (an additional line of excitation: the laser 561nm, and 6 detectors for the BV on the Violet laser).
Furthermore, the version 8 of the software BD FACS DIVA was installed on this device :
- The version 8 allows  Overlays, Undo / Redo and Copy / Paste of all the elements of  the Worksheet.
- This version introduces a new very innovative application: the Index Sorting. It allows the users who sort and deposit a unique cell in  multi-well plate (96 wells for example), to examine the phenotype of every collected cell, to identify them on graphs and edit a complete report for every cell. 

Localisation : Broussais, LRB119 – contact : jerome.megret@inserm.fr

 

  • Cell Imaging --> Microscope Leica TCS STED gated-SP8-3X

The imaging platform has just acquired a new confocal microscope equipped with STED (Stimulated Emission Depletion) technology.
The STED is a super-resolution method which breaks the optical diffraction limit and achieves resolutions of the order of a few tens of nanometers.
The main strengths of this technology are :
1 . Lateral resolution without any additional post- processing below 50 nm due to the vortex " donut "
2 . Intrinsic confocal optical sectioning , allowing the acquisition of three-dimensional structures
3 . Fast image acquisition of several images per second
4 . Live- imaging capabilities by using either fluorescent proteins or other fluorescent tags

 

Seminars
Monday 19 of November, 2018

Novel CRISPR/Cas9-based approaches for the treatment of sickle cell disease
Vasco MENEGHINI
Laboratoire chromatine et régulation génique au cours du développement, Institut Imagine

Institut des maladies génétiques IMAGINE
auditorium, 6ème étage
24, bd du Montparnasse 75015 Paris

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Seminars
Friday 23 of November, 2018

From intracellular trafficking to intercellular communication: a journey in the skin
Cédric DELEVOYE
Département de Biologie Cellulaire et Cancer (CRNS UMR144) de l'Institut Curie

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by E. Morel

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Seminars
Wednesday 28 of November, 2018

Bionano Genome Mapping: High-Throughput Mapping of Structural Variation in Cancer and Genetic Disease
Sven BOCKLANDT
Director, Scientific Affairs, Bionano Genomics

Institut des maladies génétiques IMAGINE
salle 330, 3ème étage
24, bd du Montparnasse 75015 Paris

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Seminars
Friday 30 of November, 2018

Save the β-cell! A journey from pathway discovery to preclinical studies
Kathrin MAEDLER
University of Bremen, Allemagne

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by P. van Endert

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Seminars
Monday 3rd of December, 2018

Dissecting mechanisms of tumor immunosurveillance and immunotherapies at the single cell level
Philippe BOUSSO
Institut Pasteur

Institut des maladies génétiques IMAGINE
auditorium, 6ème étage
24, bd du Montparnasse 75015 Paris

Invited by Julien Zuber

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Seminars
Friday 14 of December, 2018

Innate immune memory in microglia shapes neurological disease hallmarks
Jonas NEHER
German Centre for Neurodegenerative Diseases (DZNE), Tübingen

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by P. van Endert

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