IFR Necker-Enfants Malades (IRNEM – IFR 94) was replaced with SFR Necker on January 1st, 2014. It is officially recognized by four public institutions, namely AP-HP, CNRS, INSERM and University Paris Descartes. SFR Necker is entrusted with the development and optimization of technological platforms, as well as contributing to scientific activities on Necker/Broussais site.

To date, 11 platforms are operational and some of them are accessible to researchers and partners institutions from Ile de France Region.


SFR Necker future events

  • Executive committee meeting: September 20th at Broussais
  • SFR Necker annual seminar:  November 29th 2018 at Imagine

 


Recent acquisitions

  • Cytometry core facility, site Broussais --> Spectral analyzer SP6800 SONY

The SP6800 is a very intuitive analyzer cytometer, intended for the autonomous users.
The spectral technology optimizes the sensibility and improves the detection of low signals by collecting the photons from 420nm to 800nm. The spectral technology simplifies the design of multi-panels  colors, by eliminating band-pass filters and the conventional matrices of compensation.
The implemented spectral deconvolution makes the simpler and easier analysis by separating the individual spectral imprints of each fluorochrome. We obtain a more complete image of the rare populations. The complexities associated with the work with fluorescent proteins and fluorochromes excited by several lasers are decreased

Localisation : Broussais, LRB119 – contact : corinne.cordier@inserm.fr

 

  • Cytometry core facility, site Broussais --> Upgrade of the cell sorter in version BD FACS Aria IIIu

This new version improves the systems fluidic, computing and especially optics (an additional line of excitation: the laser 561nm, and 6 detectors for the BV on the Violet laser).
Furthermore, the version 8 of the software BD FACS DIVA was installed on this device :
- The version 8 allows  Overlays, Undo / Redo and Copy / Paste of all the elements of  the Worksheet.
- This version introduces a new very innovative application: the Index Sorting. It allows the users who sort and deposit a unique cell in  multi-well plate (96 wells for example), to examine the phenotype of every collected cell, to identify them on graphs and edit a complete report for every cell. 

Localisation : Broussais, LRB119 – contact : jerome.megret@inserm.fr

 

  • Cell Imaging --> Microscope Leica TCS STED gated-SP8-3X

The imaging platform has just acquired a new confocal microscope equipped with STED (Stimulated Emission Depletion) technology.
The STED is a super-resolution method which breaks the optical diffraction limit and achieves resolutions of the order of a few tens of nanometers.
The main strengths of this technology are :
1 . Lateral resolution without any additional post- processing below 50 nm due to the vortex " donut "
2 . Intrinsic confocal optical sectioning , allowing the acquisition of three-dimensional structures
3 . Fast image acquisition of several images per second
4 . Live- imaging capabilities by using either fluorescent proteins or other fluorescent tags

 

Seminars
Friday 21 of September, 2018

LRH1/NR5A2 activation promotes a cross talk between immune and islet cells reversing Type 1 diabetes mellitus
Benoit GAUTHIER
Andalusian Molecular Biology and Regenerative Medicine Centre, Seville

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by P. van Endert

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Seminars
Friday 28 of September, 2018

Mechanotransduction in development: from mesoderm mechanotransductive evolutionary origins to tumourigenic mechanical induction
Emmanuel FARGE
Mechanics and Genetics of Embryonic and Tumoral Development group, UMR 168 Physico-Chimie, Institut Curie, Paris

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by F. Terzi et M. Pontoglio

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Seminars
Friday 5 of October, 2018

Signatures of vaccine and infection response in adaptive immune repertoires from high throughput sequencing data
Alexandra WALCZAK
Laboratoire de physique théorique, ENS, Paris

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by J. Davoust

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Seminars
Friday 12 of October, 2018

The role of Stat3 and clun in Prostate Cancer
Lukas KENNER
Medical University of Vienna, Autriche

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by V. Goffin

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Seminars
Friday 19 of October, 2018

Hepatic steatosis, a 4500 years old metabolic story
Pascal FERRE
Centre de Recherche des Cordeliers, Paris

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by J.-C. Weill

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Seminars
Friday 26 of October, 2018

Senescence controls prostatic neoplasia driven by Pten loss
Daniel METZGER
Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Strasbourg

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by V. Goffin

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