IFR Necker-Enfants Malades (IRNEM – IFR 94) was replaced with SFR Necker on January 1st, 2014. It is officially recognized by four public institutions, namely AP-HP, CNRS, INSERM and University Paris Descartes. SFR Necker is entrusted with the development and optimization of technological platforms, as well as contributing to scientific activities on Necker/Broussais site.

To date, 12 platforms are operational and some of them are accessible to researchers and partners institutions from Ile de France Region.


SFR Necker future events

  • Executive committee meeting: Thursday  28 September 2017 at Imagine
  • SFR Necker annual seminar: Thursday 9 November, 2017 at Imagine, auditorium, 6 floor

 


Recent acquisitions

  • Cytometry core facility, site Broussais --> Spectral analyzer SP6800 SONY

The SP6800 is a very intuitive analyzer cytometer, intended for the autonomous users.
The spectral technology optimizes the sensibility and improves the detection of low signals by collecting the photons from 420nm to 800nm. The spectral technology simplifies the design of multi-panels  colors, by eliminating band-pass filters and the conventional matrices of compensation.
The implemented spectral deconvolution makes the simpler and easier analysis by separating the individual spectral imprints of each fluorochrome. We obtain a more complete image of the rare populations. The complexities associated with the work with fluorescent proteins and fluorochromes excited by several lasers are decreased

Localisation : Broussais, LRB119 – contact : corinne.cordier@inserm.fr

 

  • Cytometry core facility, site Broussais --> Upgrade of the cell sorter in version BD FACS Aria IIIu

This new version improves the systems fluidic, computing and especially optics (an additional line of excitation: the laser 561nm, and 6 detectors for the BV on the Violet laser).
Furthermore, the version 8 of the software BD FACS DIVA was installed on this device :
- The version 8 allows  Overlays, Undo / Redo and Copy / Paste of all the elements of  the Worksheet.
- This version introduces a new very innovative application: the Index Sorting. It allows the users who sort and deposit a unique cell in  multi-well plate (96 wells for example), to examine the phenotype of every collected cell, to identify them on graphs and edit a complete report for every cell. 

Localisation : Broussais, LRB119 – contact : jerome.megret@inserm.fr

 

  • Cell Imaging --> Microscope Leica TCS STED gated-SP8-3X

The imaging platform has just acquired a new confocal microscope equipped with STED (Stimulated Emission Depletion) technology.
The STED is a super-resolution method which breaks the optical diffraction limit and achieves resolutions of the order of a few tens of nanometers.
The main strengths of this technology are :
1 . Lateral resolution without any additional post- processing below 50 nm due to the vortex " donut "
2 . Intrinsic confocal optical sectioning , allowing the acquisition of three-dimensional structures
3 . Fast image acquisition of several images per second
4 . Live- imaging capabilities by using either fluorescent proteins or other fluorescent tags

 

Seminars
Friday 22 of September, 2017

NF-kappaB Signalling in Dendritic Cells: A tale of two kinases
Toby LAWRENCE
CIML, Marseille

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by P. van Endert

View the complete article...
Seminars
Friday 29 of September, 2017

What activates the ER-stress response? Unfolded proteins or aberrant lipid comprositions?
Robert ERNST
– Saarland University School of Medicine, Homburg, Germany

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by M. Pende

View the complete article...
Seminars
Monday 2nd of October, 2017

Transient moving organizers: life and death in cortical development, evolution and pathology
Alessandra PIERANI
Institut Imagine

Institut des maladies génétiques IMAGINE
Auditorium, 6ème étage
24, bd du Montparnasse 75015 Paris

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Seminars
Friday 6 of October, 2017

Decoding immune responses to cancer and infection using intravital imaging
Philippe BOUSSO
Institut Pasteur, Paris

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by P. van Endert

View the complete article...
Seminars
Monday 9 of October, 2017

From intracellular antibodies to small molecule inhibitors of mutant KRAS
Terry RABBITTS
Weatherall Institute of Molecular Medicine, University of Oxford

Institut des maladies génétiques IMAGINE
Auditorium, 6ème étage
24, bd du Montparnasse 75015 Paris

Invited by M. Cavazzana

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Seminars
Friday 13 of October, 2017

Development of multiciliated ependymal cells in the mammalian brain
Nathalie SPASSKY

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by M. Pende

View the complete article...