IFR Necker-Enfants Malades (IRNEM – IFR 94) was replaced with SFR Necker on January 1st, 2014. It is officially recognized by four public institutions, namely AP-HP, CNRS, INSERM and University Paris Descartes. SFR Necker is entrusted with the development and optimization of technological platforms, as well as contributing to scientific activities on Necker/Broussais site.

To date, 11 platforms are operational and some of them are accessible to researchers and partners institutions from Ile de France Region.


SFR Necker future events

  • Executive committee meeting: Thursday 18 January 2018 at Imagine
  • SFR Necker annual seminar: Thursday 9 November, 2017 at Imagine, auditorium, 6 floor

 


Recent acquisitions

  • Cytometry core facility, site Broussais --> Spectral analyzer SP6800 SONY

The SP6800 is a very intuitive analyzer cytometer, intended for the autonomous users.
The spectral technology optimizes the sensibility and improves the detection of low signals by collecting the photons from 420nm to 800nm. The spectral technology simplifies the design of multi-panels  colors, by eliminating band-pass filters and the conventional matrices of compensation.
The implemented spectral deconvolution makes the simpler and easier analysis by separating the individual spectral imprints of each fluorochrome. We obtain a more complete image of the rare populations. The complexities associated with the work with fluorescent proteins and fluorochromes excited by several lasers are decreased

Localisation : Broussais, LRB119 – contact : corinne.cordier@inserm.fr

 

  • Cytometry core facility, site Broussais --> Upgrade of the cell sorter in version BD FACS Aria IIIu

This new version improves the systems fluidic, computing and especially optics (an additional line of excitation: the laser 561nm, and 6 detectors for the BV on the Violet laser).
Furthermore, the version 8 of the software BD FACS DIVA was installed on this device :
- The version 8 allows  Overlays, Undo / Redo and Copy / Paste of all the elements of  the Worksheet.
- This version introduces a new very innovative application: the Index Sorting. It allows the users who sort and deposit a unique cell in  multi-well plate (96 wells for example), to examine the phenotype of every collected cell, to identify them on graphs and edit a complete report for every cell. 

Localisation : Broussais, LRB119 – contact : jerome.megret@inserm.fr

 

  • Cell Imaging --> Microscope Leica TCS STED gated-SP8-3X

The imaging platform has just acquired a new confocal microscope equipped with STED (Stimulated Emission Depletion) technology.
The STED is a super-resolution method which breaks the optical diffraction limit and achieves resolutions of the order of a few tens of nanometers.
The main strengths of this technology are :
1 . Lateral resolution without any additional post- processing below 50 nm due to the vortex " donut "
2 . Intrinsic confocal optical sectioning , allowing the acquisition of three-dimensional structures
3 . Fast image acquisition of several images per second
4 . Live- imaging capabilities by using either fluorescent proteins or other fluorescent tags

 

Seminars
Friday 17 of November, 2017

How do XCR1+ dendritic cells promote lymphocyte activation in response to infections and cancer
Karine CROZAT
CIML, Marseille

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by P. van Endert

View the complete article...
Seminars
Monday 20 of November, 2017

Gene therapy for neuromuscular diseases: opportunities and challenges
Fulvio MAVILIO
Department of Life Sciences, University of Modena and Reggio Emilia, Italy

Institut des maladies génétiques IMAGINE
Auditorium, 6ème étage
24, bd du Montparnasse 75015 Paris

Invited by A. Miccio

View the complete article...
Seminars
Wednesday 22 of November, 2017

Adapative immune response to Zika virus
Marcelo BOZZA
Laboratory of Inflammation and Immunity, Federal University of Rio de Janeiro

Hôpital Necker Enfants Malades
Bâtiment Hamburger H2,
5ème étage, pièce CNRS

Invited by B. Manoury

View the complete article...
Seminars
Friday 24 of November, 2017

Recent advances in Gene Editing technology – high efficiency repair of multiple CF-causing mutations
Patrick HARRISSON
University College, Cork, Irlande

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by I. Sermet

View the complete article...
Seminars
Friday 1st of December, 2017

Molecular Regulation, Biological Functions, and therapeutic Applications of Beclin 1-Dependent Autophagy
Beth LEVINE
Univ. of Texas Medical Center, Dallas, USA

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by P. Codogno

View the complete article...
Seminars
Friday 15 of December, 2017

« Intracellular Salmonella persisters
Sophie HELAINE
Imperial College, Londres, UK

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by E. Bille

View the complete article...