IFR Necker-Enfants Malades (IRNEM – IFR 94) was replaced with SFR Necker on January 1st, 2014. It is officially recognized by four public institutions, namely AP-HP, CNRS, INSERM and University Paris Descartes. SFR Necker is entrusted with the development and optimization of technological platforms, as well as contributing to scientific activities on Necker/Broussais site.

To date, 11 platforms are operational and some of them are accessible to researchers and partners institutions from Ile de France Region.


SFR Necker future events

  • Executive committee meeting: Thursday 18 January 2018 at Imagine
  • SFR Necker annual seminar:  November, 2018 at Imagine

 


Recent acquisitions

  • Cytometry core facility, site Broussais --> Spectral analyzer SP6800 SONY

The SP6800 is a very intuitive analyzer cytometer, intended for the autonomous users.
The spectral technology optimizes the sensibility and improves the detection of low signals by collecting the photons from 420nm to 800nm. The spectral technology simplifies the design of multi-panels  colors, by eliminating band-pass filters and the conventional matrices of compensation.
The implemented spectral deconvolution makes the simpler and easier analysis by separating the individual spectral imprints of each fluorochrome. We obtain a more complete image of the rare populations. The complexities associated with the work with fluorescent proteins and fluorochromes excited by several lasers are decreased

Localisation : Broussais, LRB119 – contact : corinne.cordier@inserm.fr

 

  • Cytometry core facility, site Broussais --> Upgrade of the cell sorter in version BD FACS Aria IIIu

This new version improves the systems fluidic, computing and especially optics (an additional line of excitation: the laser 561nm, and 6 detectors for the BV on the Violet laser).
Furthermore, the version 8 of the software BD FACS DIVA was installed on this device :
- The version 8 allows  Overlays, Undo / Redo and Copy / Paste of all the elements of  the Worksheet.
- This version introduces a new very innovative application: the Index Sorting. It allows the users who sort and deposit a unique cell in  multi-well plate (96 wells for example), to examine the phenotype of every collected cell, to identify them on graphs and edit a complete report for every cell. 

Localisation : Broussais, LRB119 – contact : jerome.megret@inserm.fr

 

  • Cell Imaging --> Microscope Leica TCS STED gated-SP8-3X

The imaging platform has just acquired a new confocal microscope equipped with STED (Stimulated Emission Depletion) technology.
The STED is a super-resolution method which breaks the optical diffraction limit and achieves resolutions of the order of a few tens of nanometers.
The main strengths of this technology are :
1 . Lateral resolution without any additional post- processing below 50 nm due to the vortex " donut "
2 . Intrinsic confocal optical sectioning , allowing the acquisition of three-dimensional structures
3 . Fast image acquisition of several images per second
4 . Live- imaging capabilities by using either fluorescent proteins or other fluorescent tags

 

Seminars
Friday 26 of January, 2018

The infectious origin of childhood B cell precursor Acute Lymphoblastic Leukemia
Julia HAUER
Heinrich Heine University, Düsseldorf, Allemagne

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by E. Macintyre

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Seminars
Friday 9 of February, 2018

Lymph node macrophages and antibody export
Marc BAJENOFF

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by S. Fillatreau

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Seminars
Friday 16 of February, 2018

Mitophagy modulation as a potential therapeutic target for neurodegenerative diseases
Hélène PLUN-FAVREAU
UCL Institute of Neurology, Londres

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by T. Wai

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Seminars
Friday 16 of March, 2018

What activates the ER-stress response? Unfolded proteins or aberrant lipid compositions?
Jesper Velgaard OLSEN
Novo Nordisk Foundation Center for Protein Research, Copenhague, Danemark

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by F. Terzi

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Seminars
Friday 30 of March, 2018

The NEAT journey from hypothalamus to city-scape
Pr James LEVINE
Président Fondation IPSEN, Paris

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by V. Goffin

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Seminars
Friday 13 of April, 2018

Mitochondrial metabolism and cancer: oncometabolites and beyond
Christian FREZZA
University of Cambridge, Cambridge, UK

Site Broussais
Amphithéâtre Leriche - Porte 10
14 rue Maria Helena Vieira Da Silva
75014 Paris

Invited by M. Pende

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